GLOBAL screening of mud crab (Scylla paramamosain) innate immunity in embryogenesis
نویسندگان
چکیده
منابع مشابه
Identification, Characterization, and Functional Analysis of Tube and Pelle Homologs in the Mud Crab Scylla paramamosain
Tube and Pelle are essential components in Drosophila Toll signaling pathway. In this study, we characterized a pair of crustacean homologs of Tube and Pelle in Scylla paramamosain, namely, SpTube and SpPelle, and analyzed their immune functions. The full-length cDNA of SpTube had 2052 bp with a 1578 bp open reading frame (ORF) encoding a protein with 525 aa. A death domain (DD) and a kinase do...
متن کاملCharacterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain
Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY ...
متن کاملCharacterization of microRNAs in Mud Crab Scylla paramamosain under Vibrio parahaemolyticus Infection
BACKGROUND Infection of bacterial Vibrio parahaemolyticus is common in mud crab farms. However, the mechanisms of the crab's response to pathogenic V. parahaemolyticus infection are not fully understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs that function as regulators of gene expression and play essential roles in various biological processes. To understand the underlying mech...
متن کاملMolecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain.
The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx1) showing high identity to the first Tr...
متن کاملRapid isolation and characterization of polymorphic microsatellite loci in the mud crab, Scylla paramamosain (Portunidae).
Scylla paramamosain is a widespread and commercially important species of coastal marine crab. We identified 13 polymorphic microsatellite loci from a genome library constructed with 5'-anchored PCR method. Thirty-two S. paramamosain from the East China Sea were used to analyze the characteristics of these loci. The number of alleles per locus ranged from 3 to 8, with a mean of 5.923. Observed ...
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ژورنال
عنوان ژورنال: Fish & Shellfish Immunology
سال: 2016
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2016.04.118